基本情况

黄昊文，男，1969年生，博士，教授，博士生导师，现任湖南科技大学教育部重点实验室副主任。毕业于中国科学院化学研究所，美国德克萨斯大学圣安东尼奥分校访问学者(2016.3-2017.3), 2009、2011年两度赴香港城市大学进行合作研究。连续主持三项国家自然科学基金，已完成国家自然科学基金、湖南省自然科学基金、湖南省教育厅青年项目、国家重点实验室开放课题等多项研究项目。以第一作者或通讯联系人身份在《Biomaterials》、《Analytical Chemistry》、《Chemical Communications》、《Biosensor and Bioelectronics》、《ACS Applied Materials and Interfaces》等国际刊物上发表科研论文40余篇。已申请国家发明专利15项，获湖南省2016年度自然科学二等奖（排名第一）。

主持课题

[1] 国家自然科学基金项目：基于碳点及复合物模拟酶构建食品中金黄色葡萄球菌及肠毒素的现场、快速检测方法研究（21675049），2017.01-2020.12；

[2] 国家自然科学基金项目：可控纳米结构表面等离子体共振生物传感器的构建及肝癌早期检测研究（21375036），2014.01-2017.12；

[3] 核壳型纳米棒构建生物传感器阵列的研究（项目编号：21075035），2011.01-2013.12;

[4] 湖南省自然科学基金项目：基于双重放大信号策略构建检测致病菌及毒素的高灵敏度可视化方法（2020JJ4289）, 2020.01-2023.12；

[5] 湖南省自然科学基金项目：可控金纳米复合结构表面等离子体共振生物传感器的构建研究(14JJ70054), 2014.01-2016.12;

[6] 湖南省高校创新平台开放基金项目：基于金纳米棒的一维自组装及解组装构建生物传感方法研究(13K090), 2014.01-2016.12;

[7] 湖南省自然科学基金项目：壳层包覆金纳米棒的生物传感器研制与应用(10JJ5004), 2010.01-2012.12;

[8] 高性能陶瓷和超微结构国家重点实验室开放课题：核壳型纳米棒构建生物传感器的研究（SKL201006SIC）, 2011.01-2012.12；

[9] 中国科学院特种无机涂层重点实验室开放课题：基于核壳型纳米粒子的表面等离子体共振性质构建多通量表面分析方法的研究（KLICM-2011-09），2012.01-2013.12；

[10] 固体表面物理化学国家重点实验室：基于金纳米棒的表面分析新方法研究钙调神经磷酸酶与药物的相互作用，2008.01-2009.12；

[11] 湖南省教育厅优秀青年项目：新型生物传感器—表面等离子体共振成像仪的研制（06B28），2007.01-2009.12。

代表性论文

[1] Xiaoxue Xie, Xinyi Hua, Ziqi Wang, Xiumei Yang, Haowen Huang*. Real-Time Imaging Redox Status in Biothiols and Ferric Metabolism of Cancer Cells in Ferroptosis Based on Switched Fluorescence Response of Gold Carbon Dots, Analytical Chemistry, 2020, 92,11420-11428.

[2] Xiaoxue Xie, Zhenqi Peng, Xinyi Hua, Zhifang Wang, Keqin Deng, Xiumei Yang, Haowen Huang*. Selectively monitoring glutathione in human serum and growth-associated living cells using gold nanoclusters, Biosensors and Bioelectronics , 2020, 148, 111829.

[3] Xinyi Hua, Zhichao, Yang, Zhifang, Wang, Xiaoxue Xie, Zaichun Zhou, Xiumei Yang, Keqin Deng, Haowen, Huang*. Rapid modification of hemoglobin heme to form enhanced peroxidase-like activity for colorimetric assay, Biosensors and Bioelectronics: X , 2020, 4, 100041.

[4] Xiaoxue Xie, Zhenqi Peng, Ziqi Wang, Xinyi Hua, Zhifang Wang, Keqin Deng, Xiumei Yang, Haowen Huang*, Xuanyong Liu*. Monitoring biothiols dynamics in living cells by ratiometric fluorescent gold carbon dots, Talanta , 2020, 218, 121214.

[5] Xiaoxue Xie, Fang Tan, Aiqing Xu, Keqin Deng, Yulong Zeng, Haowen Huang*. UV-induced peroxidase-like activity of gold nanoclusters for differentiating pathogenic bacteria and detection of enterotoxin with colorimetric readout, Sensors and Actuators B: Chemical , 2019, 279, 289-297.

[6] Fang Tan, Xiaoxue Xie, Aiqing Xu, Keqin Deng, Yunlong Zeng, Xiumei Yang, Haowen Huang*. Fabricating and regulating peroxidase-like activity of eggshell membrane-templated gold nanoclusters for colorimetric detection of staphylococcal enterotoxin B, Talanta , 2019, 194, 634-642.

[7] Fang Tan, Zhifang Wang, Yan Yang, Xiaoxue Xie, Xinyi Hua, Xiumei Yang, Haowen Huang*. Facile preparation of peroxidase-like core-shell nanorods and application as platform for colorimetric determination of glucose, insulin and glucose/insulin ratio, Talanta , 2019, 204, 285-293

[8] Keqin Deng*, Xinyan Liu, Chunxiang Li, Haowen Huang*. Sensitive electrochemical sensing platform for microRNAs detection based on shortened multi-walled carbon nanotubes with high-loaded thionin, Biosensors and Bioelectronics , 2018, 117, 168-174.

[9] Fang Tan, Yan Yang, Xiaoxue Xie, Linqian Wang, Keqin Deng, Xiaodong Xia, Xiumei Yang, Haowen Huang*. Prompting peroxidase-like activity of gold nanorod composites by localized surface plasmon resonance for fast colorimetric detection of prostate specific antigen, Analyst , 2018, 143, 5038-5045.

[10] Haowen Huang*, Lanfang LiuLingyang ZhangQian ZhaoYuan ZhouShishan YuanZilong TangXuanyong Liu. Peroxidase-Like Activity of Ethylene Diamine Tetraacetic Acid and Its Application for Ultrasensitive Detection of Tumor Biomarkers and Circular Tumor Cells,AnalyticalChemistry, 2017, 8 9 , 666–672.

[11] Qian Zhao, Haowen Huang*, Lingyang Zhang, Linqian Wang, Yunlong Zeng, Xiaodong Xia, Fengping Liu, Yi Chen. Strategy To Fabricate Naked-Eye Readout Ultrasensitive Plasmonic Nanosensor Based on Enzyme Mimetic Gold Nanoclusters,AnalyticalChemistry, 2016, 88 , 1412–1418.

[12] Lingyang Zhang, Donghui Wang, Haowen Huang*, Lanfang Liu, Yuan Zhou, Xiaodong Xia, Keqin Deng, Xuanyong Liu. Preparation of Gold–Carbon Dots and Ratiometric Fluorescence Cellular Imaging，ACS Appl. Mater. Interfaces, 2016, 8 , 6646–6655.

[13] Shenna Chen, Qian Zhao, Fang Liu, Haowen Huang*, Linqian Wang, Shoujun Yi, Yunlong Zeng, and Yi Chen. Ultrasensitive determination of copper in complex biological media based on modulation of plasmonic properties of gold nanorods, Analytical Chemistry, 2013, 85 , 9142–9147.

[14] Haowen Huang*, Shenna Chen, Fang Liu, Qian Zhao, Bo Liao, Shoujun Yi, Yunlong Zeng, Multiplex plasmonic sensor for detection of different metal ions based on single type of gold nanorod, Analytical Chemistry , 2013, 85 , 2312-2319.

[15] Haowen Huang*, Fang Liu, Shenna Chen, Qian Zhao, Bo Liao, Yunfei Long, Yunlong Zeng, Xiaodong Xia, Enhanced fluorescence of chitosan based on size change of micelles and application to directly selective detecting Fe3+ in human serum, Biosensors and Bioelectronics , 2013, 42 , 539-

[16] Haowen Huang*, Caiting Qu, Xuanyong Liu, Shaowen Huang, Zhongjian Xu, Yingjie Zhu, Paul K. Chu, Amplification of localized surface plasmon resonance signals by a gold nanorod assembly and ultra-sensitive detection of mercury, Chemical Communications , 2011, 47 , 6897-6899.

[17] Haowen Huang*, Caiting Qu, Xuangyong Liu, Shaowen Huang, Zhongjian Xu, Bo Liao, Yunlong Zeng, Paul. Chu, Preparation of controllable core-shell gold nanoparticles and its application in detection of silver ions, ACS Applied Materials & Interfaces, 2011, 3, 183-190.

奖励荣誉

2016年湖南省自然科学二等奖（排名第一）

专利成果

[1] 黄昊文，谭芳，谢潇雪，许爱清，邓克勤，检测金黄色葡萄球菌及肠毒素B试剂盒的制备方法及应用，发明专利申请号：ZL201710728640.8

[2] 黄昊文，周媛，赵倩，刘兰芳，张凌阳，一种基于乙二胺四乙酸模拟酶试剂盒的制备方法及其应用，发明专利申请号：201510545849.1。

[3] 黄昊文，张凌阳，刘兰芳，周媛，一种具有催化性能的碳点制备及基于此碳点试剂盒的应用，发明专利申请号，201510545769.6。

研究方向

本课题组的研究方向为分析化学，生化分析，具体内容包括：

[1] 纳米化学/生物传感器的构建与应用；

[2] 高灵敏度可视化检测方法研究

[3] 食品安全、临床医学分析中的试剂盒、临床试纸研制等